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Amplification of early drought responses caused by volatile cues emitted from neighboring tea plants
VOCs NCED ABA 茶树
2023/4/19
Plants have developed sophisticated mechanisms to survive in dynamic environments. Plants can communicate via volatile organic compounds (VOCs) to warn neighboring plants of threats. In most cases, VO...
Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901
IS901 IS900 PCR Johne’s diseases avian tuberculosis
2015/1/26
The aim of this study was to examine the specificity of primers designed to detect the insertion element IS901 commonly used in differentiation of Mycobacterium avium complex strains. This study shows...
Specific Amplification of Microsporidian DNA F ragments Using Multiprimer PCR
Nosema Bombyx mori Silkworm pebrine transovum transmission
2009/9/10
Using a mixture of several primers, the suitability of multiprimer PCR for the early and simultaneous detection of various kinds of infectious microsporidia of silkworms was evaluated. As a result, sp...
Identification of Transferred Chicken Germ Cells in Quail Gonad and Semen by Amplification of Chicken-Specific PCR Products
microsatellite DNA chicken PCR primordial germ cells quail
2009/8/3
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No.X85538), amplified a 363bp fragment of genomic DNA in chickens (Gallu...
Genetic Diversity among Wild Resources of the Genus Boehmeria Jacq. from West China Determined Using Inter-simple Sequence Repeat and Rapid Amplification of Polymorphic DNA Markers
Boehmeria ISSR marker RAPD marker Taxonomic analysis UPGMA
2009/7/20
Ramie (Boehmeria nivea L.Gaud) is planted as an important fiber crop in China. Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used for the first time fo...
Primer sets for DNA amplification of the noncoding regions of the chloroplast genome in the grass family
grass family Poaceae noncoding region primer chloroplast genome
2009/3/11
We designed 74 primers (37 pairs) for the DNA amplification of 37 noncoding regions in the large single copy (LSC) and the small single copy region (SSC) of the chloroplast genome in the grass famil...
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
利用RACE技术扩增大豆抗病基因同源cDNA 5′末端序列Amplification of 5′ cDNA End of a Disease Resistance Gene Homolog Using RACE System from Soybean
5′-RACE 抗病基因同源序列 大豆
2007/12/31
摘要利用抗病基因保守序列筛选大豆cDNA文库，获得一抗病基因同源cDNA片段，命名为KR3-1。根据KR3-1设计两个基因特异引物（GSP 和 NGSP），分别与通用引物(UPM)和巢式通用引物（NUP）共同扩增，成功地克隆到了该基因的5′末端序列。该扩增片段长447 bp，与已知序列重叠部分为129 bp。
Abstract:Based on part of a known partial c...
Sexing Goat Embryos by PCR Amplification of X- and Y- chromosome Specific Sequence of the Amelogenin Gene
Goat Embryos Amelogenin Gene Sexing PCR
2016/4/26
The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing goat embryos. Based on the amelogenin gene located on the conservation region of X- and Y- chromoso...
Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species
Microsatellites Cross-species Amplification Mystus nemurus Pangasius micronemus Clarias batrachus
2016/4/20
The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other tha...
ISOLATION AND AMPLIFICATION BY POLYMERASE CHAIN REACTION DNA OF BABESIA MICROTI AND BABESIA DIVERGENS IN TICKS IN POLAND
Babesia microti B. divergens Ixodes ricinus
2010/12/24
Babesia microti and B. divergens, the etiological agents of human babesiosis,are transmitted by the bite of Ixodes ricinus. The purpose of this study was differentiation of those two species in ticks ...