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根据《中国食品科学技术学会团体标准工作管理办法》等规定，我学会组织起草了《葡萄球菌肠毒素测定 ELISA试剂盒法（征求意见稿）》团体标准。现公开征求意见，请于2023年8月11日前将相关意见反馈学会秘书处。
食品中苏丹红Ⅰ间接竞争ELISA方法的研究
酶联免疫吸附反应 检测
2015/12/23
建立食品中苏丹红Ⅰ免疫学检测方法。方法 本室自制获得单克隆抗体，优化反应条件，初步建立检测苏丹红Ⅰ的间接竞争ELISA方法，确定抗原包被浓度为1.00 μg/ml，包被温度为4 ℃过夜37 ℃1 h，单抗稀释倍数为1：3200，底物作用时间15 min。结果 检测方法的回归方程和相关系数为：y=-38.541x+131.1，r=0.9841；线性范围为0.053～0.92 μg/L，最低检测浓度为...
本文采用活性酯法和N,N-羰基二咪唑法分别合成了酸性红73的人工抗原,方阵滴定实验表明活性酯法所得兔抗酸性红73的Ig G更适合用于后继实验,以此为基础建立了一种虾仁中酸性红73的间接ELISA检测方法并对反应条件进行了优化。优化后,该方法的半数抑制浓度(IC50)为45.39μg/L,检测限(LOD)为5.64μg/L。交叉反应实验结果表明除苏丹红3号(0.15%)外与番红花红、苯酚红、刚果红、...
间接竞争ELISA法检测坚果类过敏原特异性研究
坚果 过敏原 ELISA 棋盘法
2016/5/19
以腰果蛋白为过敏抗原,腰果蛋白致敏的阳性小鼠血清作为一抗,羊抗鼠Ig E-HRP为二抗建立双抗体间接酶联免疫吸附实验(ELISA)体系,通过棋盘法确定腰果蛋白最佳反应条件工作浓度为15.92μg/m L,最佳一抗稀释度均为1∶400,最佳酶标二抗的稀释度为1∶1000,腰果蛋白浓度为1.99μg/m L。并用该方法分别对腰果、核桃、榛子、开心果、板栗、杏仁等六种坚果进行检测分析,未发现有交叉反应,...
AACCI Approved Methods Technical Committee Report: Collaborative Study on the Immunochemical Determination of Intact Gluten Using an R5 Sandwich ELISA
AOAC Approved Methods Technical Committee Report Immunochemical Determination Intact Gluten an R5 Sandwich ELISA
2014/5/23
In 2008, the AACC International Protein Technical Committee (now the Protein and Enzymes Technical Committee) initiated a collaborative study of a method for determining gluten in selected foods using...
The popularity of phytoestrogen food supplements of plant origin steadily increases. They are used to solve the problems related to the climacterium. Their efficiency depends on woman's capability to ...
Evidence for wheat, rye, and barley presence in gluten free foods by PCR method – comparison with ELISA method
polymerase chain reaction ELISA wheat rye barley gliadin
2014/2/26
A method of the evidence for the presence of wheat, rye, and barley in gluten free foods, based on the polymerase chain reaction (PCR), was validated. DNA was isolated from foods by chaotropic solid p...
Comparison of the results of the ELISA, histochemical, and immunohistochemical detection of soya proteins in meat products
ELISA allergen immunochemistry immunohistochemistry histochemistry
2014/2/25
This work compares the commonly used immunochemical methods for soya protein detection and alternative microscopic methods. Immunochemical methods were represented by the competitive ELISA method. His...
Diagnostic Value of ELISA Tests for the Detection of Specific Antibodies in Cats and Rabbits
ELISA diagnostic evaluation rabbit cat
2009/3/20
Two indirect ELISA tests developed for the detection of specific IgG in cats and rabbits, infected with M. canis and T. mentagrophytes , respectively, were evaluated and compared. The levels of specif...