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Genome-wide analyses of PAM-relaxed Cas9 genome editors reveal substantial off-target effects by ABE8e in rice
AM-relaxed Cas9 nucleases cytosine base editor adenine base editor off-target effect whole-genome sequencing genome editing rice
2023/7/20
PAM-relaxed Cas9 nucleases, cytosine base editors and adenine base editors are promising tools for precise genome editing in plants. However, their genome-wide off-target effects are largely unexplore...
Transcriptome and QTL analyses reveal candidate genes for fiber quality in Upland cotton
棉花 纤维发育 QTL 转录组 表达基因
2024/5/17
本研究基于前期得到的1个F2群体、1个重组自交系群体和2个永久回交群体得到了15个在多群体或者多年分稳定检测的QTL位点。基于转录组测序，在两亲本间发育10天的纤维进行差异基因鉴定，得到了780个差异基因。通过对QTL和转录组结果联合分析，在9个QTL区间内得到31个基因，其中有9个在纤维中优异表达的基因。在这9个基因中，有7个基因在纤维伸长期高量表达，2个基因在纤维次生壁加厚期高量表达。该研究为...
Genomic analyses reveal the genetic basis of early maturity and identification of loci and candidate genes in upland cotton (Gossypium hirsutum L.)
陆地棉 全基因组 重测序方法 SNP变异图谱
2024/5/17
本研究利用收集的436份国内外变异广泛的陆地棉材料，采用全基因组重测序方法，构建了群体的SNP变异图谱，通过对包含136份早熟棉资源在内的356份陆地棉材料进行深度重测序，并结合公共数据库中的80份陆地棉材料共获得了10,118,884个高质量的SNPs。利用全基因组关联分析（GWAS）鉴定到控制陆地棉早熟性的307个显著遗传位点和重要调控基因。两个早熟遗传热点区域位于 D03和A05&...
Physiological and genome-wide gene expression analyses of cold-induced leaf rolling at the seedling stage in rice (Oryza sativa L.)☆
Rice (Oryza sativa L.) Cold-induced leaf yellowing Cold-induced leaf rolling Physiological analysis
2023/4/21
Leaf rolling and discoloration are two chilling-injury symptoms that are widely used as indicators for the evaluation of cold tolerance at the seedling stage in rice. However, the difference in cold-r...
Applied Chemical and Morphological Analyses Laboratory（ACMAL）, Michigan Technological University
ACMAL Applied Chemical and Morphological Analyses Laboratory Michigan Technological University 化学工程 电子显微镜
2007/12/24
The Applied Chemical and Morphological Analyses Laboratory (ACMAL) is a university facility that houses an extensive array of electron microanalytical and x-ray instruments. The Center is managed by t...