搜索结果: 1-15 共查到“IgY”相关记录15条 . 查询时间(0.089 秒)
响应面法优化IgY抑制具核梭杆菌增殖的研究
响应面优化 具核梭杆菌 免疫球蛋白Y 增殖
2018/10/16
目的:利用响应面法研究免疫球蛋白Y(IgY)影响口腔唾液模拟环境中具核梭杆菌(F. nucleatum)的生长繁殖特性,发现F. nucleatum最终菌落数与培养时间、F. nucleatum初始接种浓度和IgY剂量的关系。方法:以F. nucleatum最终菌落数为评价指标,在单因素试验基础上,设计三因素三水平响应面实验进行优化,通过响应面法对回归方程的分析,得到各因素最佳条件,优化后的条件重...
Geomagnetism and Aeronomy activities in Italy during IGY, 1957/58
IGY Geomagnetism Aeronomy History of Geophisics Italy
2015/8/28
In 2007 several events were organized to celebrate the fiftieth anniversary of the International Geophysical Year (IGY, 1957-1958). The celebrations will last until 2009 and are taking place within di...
Expression of goat RNA binding protein (NANOS2), development and evaluation of anti-NANOS2 IgY antibodies
NANOS2 protein dairy goat
2015/7/7
. Anti-NANOS2 immunoglobulin Y
(IgY; in chicken) was developed and evaluated. NANOS2 protein sequences between mice and dairy goat were
compared using bioinformatics tools. The specific IgY t...
日本血吸虫可溶性虫卵抗原经两种免疫途径获得的鸡卵黄抗体IgY动态观察
日本血吸虫 可溶性虫卵抗原 IgY 免疫
2010/4/20
目的 对比两种免疫途径产生抗日本血吸虫可溶性虫卵抗原（SEA）的特异性IgY抗体水平动态变化。 方法 7只新西兰兔感染日本血吸虫尾蚴（1 500/只）42 d后解剖收集虫卵，制备SEA。将SEA分别经皮下和静脉注射免疫健康海蓝蛋鸡（3只/组），首次和第2次免疫间隔2周，之后每次间隔4周，共免疫5次，50 μg/（只·次）。取两组免疫前，以及首次免疫后2～18周生产的鸡蛋，用水稀释法粗提IgY，...
POLE-TO-POLE WATER BALANCE FOR THE IGY FROM AEROLOGICAL DATA
POLE-TO-POLE WATER BALANCE IGY AEROLOGICAL DATA
2009/10/29
The present study gives a discussion of the water balance on a planetary
scale during the IGY covering the mean and the seasonal conditions for
the calendar year 1958. The study includes analyses of...
Seasonal Variability in the Pole-to-Pole Water Vapor Balance During the IGY
Seasonal Variability Pole-to-Pole Water Vapor Balance IGY
2009/10/29
A study of the vertically integrated fields of water vapor and its transport on a
planetary scale derived from aerological data taken during the IGY (International
Geophysical Year) is presented. Th...
目的: 采用基因工程技术大量表达、提纯幽门螺杆菌(H pylori)细胞空泡毒素毒性片段, 和黏附素片段的融合蛋白, 以此为抗原, 制备高效价的抗VacA-HpaA蛋黄抗体(IgY). 方法: 大量培养重组菌pQE30-VacA-HpaA-DH5a, 诱导表达获得融合蛋白VacA-HpaA, Ni2+-NTA树脂纯化. 将纯化的重组蛋白免疫鸡, 水稀释法提取IgY, 硫酸铵沉淀法纯化浓缩VacA-...
Effects of Forced Molting on the IgY Concentration in Egg Yolk of Chickens
immunoglobulin yolk forced molting chicken
2009/8/3
Immunoglobulin Y (IgY) is transferred passively from maternal circulation to the yolk. The aim of this study was to determine whether the concentration of yolk IgY is affected by forced molting in h...
Stability of Chicken IgY Antibodies Freeze-Dried in the Presence of Lactose,Sucrose and Threalose
ELISA freeze-drying IgY lactose sucrose threalose
2009/7/30
Freeze-drying is used as a method to stabilize proteins. The process itself may however cause protein unfolding and denaturation, but the risk is reduced if a stabilizing agent is added prior to free...
On the Accuracy of Wind Measurements Made with the Radiotheodolite Used during the Swedish-Finnish-Swiss IGY Expedition to Spitzbergen
Accuracy Wind Measurements Radiotheodolite Used Swedish-Finnish-Swiss IGY Expedition Spitzbergen
2009/6/19
On the Accuracy of Wind Measurements Made with the Radiotheodolite Used during the Swedish-Finnish-Swiss IGY Expedition to Spitzbergen.
Production, Characterization and Use of Monoclonal Antibodies Recognizing IgY Epitopes Shared by Chicken, Turkey, Pheasant, Peafowl and Sparrow
monoclonal antibodies IgY avian Phasianidae
2009/3/17
Chicken antibodies are not only a part of immune defense but are more and more popular commercial products in form of chicken polyclonal, monoclonal or recombinant antibodies. We produced and characte...
“抗胃癌生物导向药IGY-RICINA口服制剂”的开发
胃癌 生物导向药 IgY-RicinA口服制剂 抗癌药 生物制品
2008/12/12
恶性肿瘤的治疗是当今世界性重要课题。胃癌是中国发病率最高的恶性肿瘤，严重危害人民的生命和健康，除早期胃癌可用手术疗法达到一定的治愈外，对中晚期胃癌，目前还没有满意的治疗方法和手段，是一个急待解决的重要问题。中国科学院遗传研究所101实验室在长期进行肿瘤发生机制理论研究的基础上，以胃癌为突破口，利用生物医学工程的高新技术，成功地研制出了抗胃癌及其它消化道肿瘤的生物导向药物IgY-Ricin A，并对...
抗细胞质胸苷激酶－IgY抗体的制备及肿瘤诊断组合物
抗体 胸苷激酶 肿瘤诊断
2008/10/27
该项目开发了人胸苷激酶抗体TK1-IgY抗体制备新工艺。抗原：设计了抗原决定簇的多肽作半抗原，并将这种人工化学合成的肽与载体蛋白偶联成抗原免疫母鸡后，从蛋卵黄中分离纯化了高纯度抗体。IgY抗体：IgY抗体不仅具有传统IgG多抗的效价高、制备方法简便等优点，而且还具有单抗的特异性强的特色。抗体纯化：选用了亲和层析方法制备TK1-IgY抗体，具有操作简便，生产工艺流程短等优点。224例多种肿瘤和50例...
利用鸡蛋特异性抗体IgY的免疫亲和色谱
2007/12/13
〖摘要：以牛血清白蛋白（BSA）作为抗原，利用偶联上BSA的Sepharose-4B的亲和色谱材料从鸡蛋蛋黄中一步分离特异性抗BSA抗体。经SDS-PAGE、双向免疫扩散检验，所洗脱的样品为电泳纯的特异性抗体。反之，将所得抗体再偶联到POROSHY上可分离抗原。同时考察了母鸡免疫过程中特异性抗体随时间的变化趋势。-ZY/摘要〗〖WX/文献〗1Scouten.W.H.AffinityChromato...