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Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR
gene stability reference genes quantitative PCR Sus scrofa age categories of pigs muscle tissue
2015/6/9
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each exp...
Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
Cryptosporidium parvum oocysts spiked water and stool water filtration
2015/4/16
Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally r...
Mycobacterium avium subsp. paratuberculosis in powdered infant milk: F57 competitive real time PCR
Crohn's disease public health food safety environmental risk
2015/4/15
Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More t...
Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report
Mycobacterium avium complex mycobacteriosis qPCR IS1245 zoonosis
2015/4/15
This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium...
Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR
chicken mycobacteriosis non-tuberculous mycobacteria
2015/4/7
Mycobacterium avium subsp. avium (MAA) is the etiologic agent of avian tuberculosis, a chronic contagious disease described in a wide variety of domestic and wild bird species. The aims of this study ...
Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report
MAP Johne's disease faeces
2015/4/7
The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. Thi...
Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
bovine viral diarrhea virus RNA real-time RT-PCR SYBR Green I
2015/3/13
Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR wa...
本研究旨在揭示感染JSRV病羊体内检测不到循环抗体的免疫学机理。用地高辛（DIG）标记制备enJSRV-env探针，原位杂交法检测妊娠70 d的绵羊胎儿（免疫器官初步形成期）、妊娠130 d胎儿（即将出生的）和出生7日龄羔羊的胸腺、脾脏、肠淋巴结和肺脏内enJSRV mRNA表达情况。并利用Real-Time PCR法，对enJSRV mRNA在以上组织中的表达进行定量分析。结果表明，在所检测组织...
利用SYBR Green建立了检测种特异性衣原体的Real-Time PCR方法。本方法应用衣原体种特异性的高度保守特异引物，能够扩增627 bp特异片段；使用定量标准基因组DNA，本方法能准确检测最少250 fg衣原体DNA。Real-Time PCR方法与免疫荧光方法的检测结果表明：检测4种衣原体临床样本，Real-Time PCR敏感性均在96%~98%；特异性均为100%；这两种方法符合率...