搜索结果: 1-15 共查到“畜牧学 IGF-I”相关记录15条 . 查询时间(0.096 秒)
旨在构建干扰胰岛素样生长因子(insulin-like growth factor-II,IGF-II)的重组质粒载体,研究IGF-II对牦牛睾丸支持细胞的影响。本研究设计并合成针对牦牛IGF-II的shRNA寡核苷酸,并将其克隆至pLKO.1质粒载体上,转染牦牛睾丸支持细胞后经嘌呤霉素筛选获得稳定的细胞株,并采用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹法检测IGF-II基因和蛋白的表达...
本研究旨在克隆牦牛(Bos grunniens)Cyclin D1基因的CDS序列,分析其生物学特性,研究胰岛素样生长因子-I (Insulin-like growth factor-I,IGF-I)对Cyclin D1 mRNA和蛋白在睾丸支持细胞(Sertoli cell,SC)中表达的影响。采集5~8月龄健康牦牛的睾丸样品进行SC的分离培养,用RT-PCR技术克隆牦牛Cyclin D1基因,...
太湖鹅与皖西白鹅胸肌IGF-I 和MSTN的表达及其与屠宰性状相关性分析
肌肉 IGF-I MSTN 屠宰
2015/5/15
本研究旨在探讨IGF-I、MSTN对鹅骨骼肌生长的影响。以太湖鹅和皖西白鹅为研究素材,采用多重荧光竞争性PCR和酶联免疫方法,测定鹅70日龄胸肌中IGF-I、MSTN的mRNA和蛋白表达情况,并与屠宰性能做相关性分析。结果证实,70日龄时太湖鹅体重、胸肌重极显著小于皖西白鹅,但是胸肌率没有差异,并且品种间在体重和胸肌重上存在性别差异;胸肌IGF-I和MSTN的mRNA、蛋白表达均无品种差异,并且在...
Changes of Hypothalamic GnRH-I, POMC and NPY mRNA Expression and Serum IGF-I and Leptin Concentrations during Maturation of Shaoxing Ducks (Anas platyrhynchos)
Changes of Hypothalamic GnRH-I, POMC and NPY mRNA Expression and Serum IGF-I and Leptin Concentrations during Maturation of Shaoxing Ducks (Anas platyrhynchos)
2016/5/6
Sexual maturity in poultry is controlled by a complex neural circuit located in the basal forebrain, which integrates the central and peripheral signals to activate hypothalamic gonadotrophin-releasin...
Effects of Volatile Fatty Acids on IGF-I, IGFBP-3, GH, Insulin and Glucagon in Plasma, and IGF-I and IGFBP-3 in Different Tissues of Growing Sheep Nourished by Total Intragastric Infusions
Volatile Fatty Acids IGF-I IGFBP-3 Growth Hormone Insulin Glucagon Sheep
2016/5/4
Twelve SuffolkSmall-tail-Han male sheep (body weight 21-26 kg), aged four months, were used to study the effects of volatile fatty acids (VFA) on IGF-I (insulin-like growth factor-I) , IGFBP-3...
从溆浦鹅(Xupu geese)的腿肌中抽提总RNA,用两步法RT-PCR扩增出MSTN基因的cDNA编码序列,以pGEM-T Vector为载体,将该片段克隆到大肠杆菌(Escherichia coli)中。通过筛选阳性克隆,双酶切鉴定后测序;以MSTN基因的克隆为基础,以β-actin为内标,构建优化的半定量RTPCR方法,研究高、中、低(A:13.38 MJ/kg、B:12.13 MJ/k...
【目的】采用反转录-聚合酶链反应(RT-PCR)技术,克隆朗德鹅肌肉生成抑制因子基因(MSTN),研究MSTN基因表达和日粮能量及其部分血清激素含量的相互关系,了解MSTN的功能,为水禽分子营养和分子育种提供基础资料。【方法】从朗德鹅(Anser anser)的腿肌中抽提总RNA,用两步法RT-PCR扩增出MSTN基因的cDNA编码序列,以pGEM-T Vector为载体,将该片段克隆到大肠杆菌(...
应用LH-RH免疫和IGF-1诱导技术提高东北梅花鹿鹿茸产量的研究
鹿茸 IGF-1诱导 LH-RH免疫
2008/9/10
该项目筛选出应用LHRH-B和LHRH-D免疫雄性梅花鹿的高效弗氏佐剂;筛选出仅处理一次就可以在生茸后期获得良好免疫和生长再生茸效果的八肽LHRH-D免疫制剂,建立了相应的免疫程序;应用八肽LHRH-D免疫制剂,可以使6月中旬锯头茬茸的东北梅花鹿再生茸生长时间平均为74.2天,再生茸生长率达到75%,平均增产312.4克;东北梅花鹿每周口服一次半胱胺,服用3周,可以使血清IGF-1水平比对照...
GNRHR、IGF-1基因对文昌鸡繁殖性状的遗传效应分析
遗传效应 繁殖性状 文昌鸡 GNRHR、IGF-1基因
2008/4/1
以控制文昌鸡繁殖性状的促性腺激素释放激素受体基因 (GNRHR) 和胰岛素样生长因子1基因 (IGF-1) 为候选基因,采用PCR-RFLP技术检测GNRHR基因和IGF-1基因在文昌鸡中的单核苷酸多态性(SNP),同时对这2个基因与文昌鸡繁殖性状的相关性进行了研究。结果表明,GNRHR基因内含子1的537 bp位置有C→T碱基的变异,在文昌鸡中检测到AA、Aa、aa 3种基因型, A等位基因...
以180只3个品系的温岭草鸡为材料,采用PCR-RFLP方法测定了IGF-Ⅰ基因的3个SNPs座位,同时分析了它们对屠体性状的遗传效应。结果显示:PstⅠ、HinfⅠ和TaqⅠ识别座位分别发生T→C、C→A和C→T突变,每个SNPs座位各出现了3种基因型,其中A系在3个座位上均处于Hardy-Weinberg平衡状态(Ρ>0.05)。方差分析显示每个座位基因型对部分屠体性状都有极显著或显著的差异(...
二花脸猪和大白猪睾丸IGF-I和IGF-IR基因表达发育变化比较
猪 睾丸 胰岛素样生长因子I 胰岛素样生长因子I型受体 发育性变化
2013/12/27
为了研究生长轴激素对猪繁殖性能发育的影响,随机选取0、3、20、30、90、120、180日龄纯种二花脸公猪和大白公猪各4头,屠宰并采取睾丸组织样,以18S rRNA为内标,用相对定量RT-PCR法研究睾丸IGF-I和IGF-IR mRNA的表达及发育性变化。结果表明,二花脸猪与大白猪睾丸IGF-I mRNA表达的发育模式在30日龄前完全相同,即随着日龄的增加而显著增加 (P﹤0.01);二花脸猪...
选择经同期发情且同期受孕,妊娠90 d的健康蒙古绵羊100只,分为代谢能(ME)0.175 MJ/(kgw0.75·d)(RG1)、0.33 MJ/(kgw0.75·d)(RG2)以及自由采食对照组(CG)3个营养水平处理组,研究妊娠后期不同营养水平限制饲养对母羊分娩后生理补偿期内血中GH、IGF-I、T3、T4和INS浓度的影响。结果表明:母羊从分娩后10 d开始,各限制组RG1和RG2组GH浓...
Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
epidermal growth factor insulin-like growth factor 1 oocyte ageing pig
2015/6/1
There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many pos...
Nutritional and Tissue Specificity of IGF-I and IGFBP-2 Gene Expression in Growing Chickens - A Review
IGF-I IGFBP-2 Gene Expression Nutrition Tissue Specificity Chickens
2016/4/19
Nutritional regulation of gene expression associated with growth and feeding behavior in avian species can become an important technique to improve poultry production according to the supply of nutrie...
Association among Egg Productivity, Granulosa Layer IGF-I, and Ovarian IGF-I in Korean Native Ogol Chicken
IGF-I Egg productivity Ovary Follicle KNOC
2016/4/12
There exists considerable evidence that insulin-like growth factor-I (IGF-I) is involved in the regulation of ovulation rate and follicle development. IGF-I is believed to modulate the effects of gona...