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119 strains of lactic acid bacteria from Chinese pickle were tested for production of antimicrobial compounds (bacteriocins). Among them, strain C8 showed strongly antibacterial activity against Staph...
A novel collagenase-producing bacterium has been isolated and identified as Bacillus cereus MBL13. From the culture supernatant of B. cereus MBL13 grown on bone collagen as the sole carbon and nitro...
An alkali-stable β-amylase was purified from Nagaimo, a cultivar of yam (Dioscorea opposita Thunb.) by hexadecyltrimethylammonium bromide treatment, ammonium sulfate fractionation, and two-step column...
An exo-1,5-α-L-arabinanase was purified as an electrophoretically homogenous protein from a liquid culture of Aspergillus sojae. The molecular mass of the purified enzyme was estimated to be 41 kDa by...
An alkaliphilic actinomycete, Nocardiopsis sp. strain TOA-1, produced extracelluar maltotriose-producing amylases. Two amylases (AmyA-1 and AmyA-2) were purified to homogeneity by three steps of chrom...
An expression plasmid containing the aglA gene encoding Aspergillus niger GN-3 α-glucosidase was constructed and inserted into Emericella nidulans JCM10259. The transformant secreted about 61 mg/L of ...
Isoprimeverose-producing oligoxyloglucan hydrolase (IPase; EC 3.2.1.120) is a unique β-glycosidase that cleaves xyloglucan oligosaccharides at the non-reducing end, producing isoprimeverose. Here, we ...
Antihypertensive peptides inhibiting angiotensin I-converting enzyme have been isolated from enzymatic hydrolysates of various food materials, but no information is available on the isolation of antih...
Whey peptides in a yogurt-like product fermented by Lactobacillus helveticus CPN4 were fractionated by a Sep-pak C-18 cartridge followed by two-step reverse-phase HPLC. The antihypertensive activity w...
A carboxypeptidase Y was purified to homogeneity from the crude cell extracts of Kluyveromyces fragilis JSB95 grown in yeast, peptone, and dextrose broth using an FPLC® system equipped with ion-ex...
The distribution of proteose-peptone component PP3 in bovine whey, milk fat globule membrane, and casein has been investigated with antibodies raised against highly purified PP3. Using Western blot an...
Bovine -lactalbumin (-LA) and bovine ß-casein (ß-CN), from milk from transgenic mice were characterized and partially purified using electrophoretic, immunoblotting, and chromatographic me...
A dipeptidase was purified to homogeneity from the cell-free extract of Lactobacillus casei ssp. casei IFPL 731 by a combination of heat treatment, hydrophobic interaction chromatography, anion-exchan...
The amino acid sequence of the bovine xanthine oxidoreductase was determined by cloning and sequencing cDNA clones encoding the enzyme. Partial amino acid sequences corresponding to 54% of the total s...
A caseolytic proteinase with low activation energy was purified to homogeneity by conventional chromatographic techniques from Lactococcus lactis ssp. lactis IAM 1198. The molecular weight of the enzy...

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