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The purpose of this activity is for students to investigate water quality, and the factors that affect it, at a local beach. This is accomplished by (1) measuring concentrations of Escherichia coli an...
DNA replication forks are frequently detained during their progression from oriC to the replication terminus due to encounters with DNA lesions or proteins blocking the way. For survival, cells deploy...
Aerobic respiration generates unavoidable reactive oxygen species (ROS, detrimental by-products that damage biological molecules. The accumulation of ROS beyond the cellular capacity for detoxificatio...
Faithful DNA replication is essential for all organisms to maintain genetic integrity. During the DNA replication, replications forks are frequently stalled or collapsed due to the encounter of DNA le...
During the course of DNA replication, replication forks often stall or collapse as they proceed from oriC to the terminus due to housekeeping types of DNA damage or proteins bound to DNA. If the DNA i...
During the course of normal DNA replication, replication forks are constantly encountering "housekeeping" types of routine damage to the DNA template that may cause the forks to stall or collapse. One...
More than 4 000 E. coli strains isolated from diarrhoeic piglets in 111 pig herds in the Czech Republic during the period 1995–2000 were examined for serogroup and virulence factors. Gene typing of th...
Biologically synthesized poly(α,L-glutamic acid) (PLGA) can be chemically modified to form monodisperse poly(γ-benzyl α,L-glutamate) (PBLG). This material shows rare smectic ordering where macromolecu...
The Hsp70 family molecular chaperones prevent protein aggregation under heat shock conditions. They are highly conserved, and have a N-terminal ATPase domain, which binds and hydrolyzes ATP, and a C-t...
The RecA protein of Escherichia coli is a central enzyme in recombination and SOS induction, both crucial DNA repair pathways. Furthermore, RecA and its homologues are found in all domains of life, fr...
Homologous recombination is generally considered a major mechanism by which cells repair many types of DNA lesions and damaged replication forks. However, if this process is left unchecked, cells ofte...
Translocation is the process by which the ribosome advances the mRNA:tRNA complex by a one-codon step during the elongation cycle of translation. In the pre-translocation state, the ribosome transitio...
Protein secretion is a selective and regulated process that is essential in all organisms. In bacteria the preprotein translocase SecA, either free in the cytosol or associated with the SecYEG translo...
Translocation is the process by which the ribosome advances the mRNA:tRNA complex by a one-codon step during the elongation cycle of translation. In the pretranslocation state, the ribosome transition...
在1L搅拌发酵罐中对含有重组质粒pRLK14的大肠杆 菌进行了培养。结果表明,采用二段培养法,于34℃增殖 殖细菌,在对数增殖后期升温到42℃进行诱导,可以高效 表达基因产品半乳品糖激酶。诱导期,醋酸浓度增长较快模 模拟实验表明,控制醋酸浓度可进一步提高半乳糖激酶产率 。

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